Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.2 (
laccase
)
4,656
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. Using isobaric tag for relative and absolute quantification (iTRAQ), 815 differential proteins were identified in the leaves of Zijuan tea, among which 20 were associated with the regulation of anthocyanin metabolism. We found that the abundances of anthocyanin synthesis-related enzymes such as chalcone synthase,
chalcone isomerase
, dihydroflavonol 4-reductase and anthocyanin synthetase, as well as anthocyanin accumulation-related UDP-glucosyl transferase and ATP-binding cassette (ABC) transporters in the purple leaves were all significantly higher than those in the green leaves. The abundances of the transcription factors bHLH and HY5, regulating anthocyanin biosynthesis at transcriptional level were also obviously higher in purple leaves than those in green leaves. In addition, bifunctional 3-dehydroquinate dehydratase and chorismate mutase in purple leaves were distinctly higher in abundance compared to green leaves, which provided sufficient phenylalanine substrate for anthocyanin synthesis. Furthermore, lignin synthesis was found to be reduced due to the lower abundances of cinnamoyl-CoA reductase 1, peroxidase 15 and
laccase
-6, which resulted in increase of intermediates flow into anthocyanin synthesis pathway. The physiological data were consistent with proteomic results. These four aspects of biosynthetic regulation contribute to anthocyanin accumulation in purple leaves of Zijuan tea.
...
PMID:Regulation of Anthocyanin Biosynthesis in Purple Leaves of Zijuan Tea (Camellia sinensis var. kitamura). 2842 49
Mixing cultures induces the biosynthesis of
laccase
in mixed cells, produces signal molecules, and regulates the production of mixed-cell metabolites. The fungal strain, which promotes
laccase
production, has been isolated and screened from the host bamboos of endophytic fungi and identified as Phoma sp. BZJ6. When the culture medium is mainly composed of soluble starch, yeast extract, and Phoma sp., the
laccase
output can reach 4,680 U/L. Nitric oxide (NO) and reactive oxygen species (ROS) were found to promote the regulation of
laccase
synthesis. Plasma membrane NAD(P)H oxidase inhibitors and NO-specific quenchers can inhibit not only the accumulation of ROS induced and NO synthesis but also the biosynthesis of
laccase
. The results indicate that the accumulation of superoxide anion radical (O
2
-
) and hydrogen peroxide (H
2
O
2
) induced by the mixed culture was partially dependent on NO. The mixed culture can also reduce the biomass, increase the synthesis of total phenolics and flavonoids, and enhance the activity of phenylalanine ammonia-lyase and
chalcone isomerase
. This phenomenon is probably the result of the activated phenylpropanoids-flavonoid pathway. Results confirmed that the mixture culture is advantageous for
laccase
production and revealed that NO, O
2
-
, and H
2
O
2
are necessary signal molecules to induce
laccase
synthesis.
...
PMID:Conditions and Regulation of Mixed Culture to Promote Shiraia bambusicola and Phoma sp. BZJ6 for Laccase Production. 2925 12
