Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.1 (
tyrosinase
)
9,065
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The organogermanium compound 3-(trihydroxygermyl)propanoic acid (
THGP
) has various biological activities. We previously reported that
THGP
forms a complex with
cis
-diol structures. L-3,4-Dihydroxyphenylalanine (L-DOPA), a precursor of melanin, contains a
cis
-diol structure in its catechol skeleton, and excessive melanin production causes skin darkening and staining. Thus, the cosmetic field is investigating substances that suppress melanin production. In this study, we investigated whether
THGP
inhibits melanin synthesis via the formation of a complex with L-DOPA using mushroom
tyrosinase
and B16 4A5 melanoma cells. The ability of
THGP
to interact with L-DOPA was analyzed by
1
H-NMR, and the influence of
THGP
and/or kojic acid on melanin synthesis was investigated. We also examined the effect of
THGP
on cytotoxicity,
tyrosinase
activity, and gene expression and found that
THGP
interacted with L-DOPA, a precursor of melanin with a
cis
-diol structure. The results also showed that
THGP
inhibited melanin synthesis, exerted a synergistic effect with kojic acid, and did not affect
tyrosinase
activity or gene expression. These results suggest that
THGP
is a useful substrate that functions as an inhibitor of melanogenesis and that its effect is enhanced by combination with kojic acid.
...
PMID:The Organogermanium Compound THGP Suppresses Melanin Synthesis via Complex Formation with L-DOPA on Mushroom Tyrosinase and in B16 4A5 Melanoma Cells. 3156 11
