Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.1 (
tyrosinase
)
9,065
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ostrinia furnacalis
, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of
Beauveria bassiana
and
Trichoderma asperellum
in order to explore larval immune response through RNA sequencing and differentially expression analysis. In vivo synergism was examined in seven proportions (
B. bassiana: T. asperellum
= 1:1, 1:2, 1:3, 1:4, 4:1, 3:1, 2:1) and in the in vitro case, two inoculation methods were applied: seed coating and soil drenching. Results revealed significant decrease in plant damage and high larval mortality in fungal treatments. Fungal isolates mediated the plant defense by increasing proline, superoxide dismutase (SOD), peroxidase (POD),
polyphenol oxidase
(
PPO
) and protease activities. Seed coating method was proved to be the most effective in case of maize endophytic colonization. In total, 59 immune-related differentially expressed genes DEGs were identified including,
cytochrome P450, heat shock protein, ABC transporter, cadherin,
peptidoglycan recognition protein
(
PGRP
), cuticlular protein
, etc. Further, transcriptomic response was confirmed by qRT-PCR. Our results concluded that, coculture of
B. bassiana
and
T. asperellum
has the synergistic potential to suppress the immune response of
O. furnacalis
and can be used as sustainable approach to induce plant resistance through activation of defense-related enzymes.
...
PMID:Synergistic Effect of
Beauveria bassiana
and
Trichoderma asperellum
to Induce Maize (
Zea mays
L.) Defense against the Asian Corn Borer,
Ostrinia furnacalis
(Lepidoptera, Crambidae) and Larval Immune Response. 3315 30
