Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.1 (
tyrosinase
)
9,065
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Tyrosinases, copper-containing monooxygenases, are widely used enzymes for industrial, medical, and environmental applications. We report the first functional surface display of Bacillus megaterium
tyrosinase
on Bacillus subtilis spores using CotE as an
anchor protein
. Flow Cytometry was used to verify surface expression of
tyrosinase
on the purified spores. Moreover,
tyrosinase
activity of the displayed enzyme on B. subtilis spores was monitored in the presence of L-tyrosine (substrate) and CuSO
4
(inducer). The stability of the spore-displayed
tyrosinase
was then evaluated after 15 days maintenance of the spores at room temperature, and no significant decrease in the enzyme activity was observed. In addition, the
tyrosinase
-expressing spores could be repeatedly used with 62% retained enzymatic activity after six times washing with Tris-HCl buffer. This genetically immobilized
tyrosinase
on the spores would make a new advance in industrial, medical, and environmental applications.
...
PMID:Surface display of bacterial tyrosinase on spores of Bacillus subtilis using CotE as an anchor protein. 2728 58
