Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.10.3.1 (
tyrosinase
)
9,065
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Our previous study has shown that VIT1 gene in Chinese vitiligo patients is de facto the
FBXO11
gene, and the silencing of that gene has an impact on the ultrastructure of melanocytes. In this study, we further identified the role of the
FBXO11
gene in melanocytes and the relationship between dilated endoplasmic reticulum (ER) and
tyrosinase
by inhibition and overexpression of
FBXO11
gene. Cell proliferation, apoptosis, cycle and migration of melanocytes were examined when the
FBXO11
gene was silenced or overexpressed. The results showed that
FBXO11
gene promoted cell proliferation and suppressed cell apoptosis, and yet had little effect on cell migration. Obvious swelling of ER was found in the cells transfected with siRNA of
FBXO11
gene. Interestingly, protein level of
tyrosinase
was extraordinarily high following inhibition of
FBXO11
gene. Further examination revealed that
tyrosinase
and calreticulin were co-localized in ER of transfected cells following siRNA of
FBXO11
gene, suggesting that
tyrosinase
could not be exported from ER effectively. Collectively, our results support the notion that
FBXO11
plays an important role in regulating proliferation and apoptosis of melanocytes, and functional export of
tyrosinase
from ER in vitiligo melanocytes.
...
PMID:The role of VIT1/FBXO11 in the regulation of apoptosis and tyrosinase export from endoplasmic reticulum in cultured melanocytes. 2051 23
