Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.1.1.37 (
malate dehydrogenase
)
4,591
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objective:
To examine protein changes in the hippocampus of APP/PS1 transgenic mice after blueberry extracts (BB) intervention.
Methods:
Eight APP/PS1 transgenic mice were randomly assigned to Alzheimer's disease (AD)+BB group (
n
=4) and AD+control group (
n
=4). After a 16-week treatment, 2-DE and MALDI-TOF-MS were used to compare the proteomic profiles of the hippocampus in the two groups and Western blot was used to confirm the important differentially expressed proteins.
Results:
Twelve proteins were differentially expressed between the two groups. Nine of them were identified. Cytochrome b-c1 complex subunit 6, beta-actin, dynamin 1, and heat shock cognate 71 were up-regulated in AD+BB group, while a-enolase, stress-induced-phosphoprotein 1,
malate dehydrogenase
(
MDH
),
MDH
1, and
T-complex protein 1 subunit beta
were down-regulated, respectively. Importantly, some of the identified proteins (e.g. dynamin 1) are known to be involved in cognitive impairment. Western blot analysis of hippocampus dynamin 1 expression confirmed the proteomic findings.
Conclusions:
The consumption of BB modulates the expression of proteins that are linked to the improvements of cognitive dysfunction in hippocampus of APP/PS1 transgenic mice.
...
PMID:Changes of hippocampus proteomic profiles after blueberry extracts supplementation in APP/PS1 transgenic mice. 2978 5
