Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.3 (HSD)
 3,464 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Development of the adrenal cortex is dependent upon the specific regulation of cellular proliferation and differentiation. Although both intra-adrenal transcription factors and extra-adrenal peptide hormones have been demonstrated as indispensable for this regulatory process, the resulting distribution of proliferating and steroidogenic cell populations in the developing adrenal cortex has not been defined. Thus, we assessed expression and colocalization of a differentiation marker (3-beta-hydroxysteroid dehydrogenase, 3beta-HSD) and a proliferation marker (5-bromo-2'-deoxyuridine (BrdU) incorporation) at the various time points (embryonic day (E) 9.5 until 2 weeks post partum) during mouse adrenal development. In addition, adrenocorticotropin-hormone (ACTH) receptor (melanocortin-2-receptor (MC2-R)) expression was examined by in situ hybridization (ISH) and co-localized with 3beta-HSD. As demonstrated by immunohistochemistry (IHC) the number of BrdU positive cells within the adrenal cortex decreased during development, whereas the number of 3beta-HSD positive cells increased. While BrdU incorporation was evident in a scattered pattern throughout the adrenal gland up to day E13.5, at later time points BrdU positive cells assembled in a discrete subcapsular compartment possibly representing the stem cell layer of the adult adrenal cortex. Interestingly, only a small percentage of proliferating cells expressed 3beta-HSD, while the majority of 3beta-HSD positive cells co-stained for MC2-R expression by means of ISH. As demonstrated by semiquantitative RT-PCR, MC2-R mRNA levels increased from E11.5 until birth, while the highest adrenal secretory protease (AsP) expression was detected at E13.5 with a decrease thereafter. Taken together, these findings are in accordance with the concept of distinct cell populations present during adrenocortical development with a highly proliferative phenotype or differentiated steroidogenic properties.
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PMID:Expression and spatio-temporal distribution of differentiation and proliferation markers during mouse adrenal development. 1692 Apr 5

Brain can synthesize steroids de novo from cholesterol and this biochemical feature is a conserved property of vertebrates. There is growing evidence indicating that neurosteroids might participate in sexual differentiation of the brain. Therefore, in this study we investigated the presence, the sex differences, and the development-dependent variation of mRNAs coding for key neurosteroidogenic enzymes, namely cytochrome P450 side-chain cleavage enzyme (P450scc), 3beta-hydroxysteroid-dehydrogenase/Delta5-Delta4-isomerase (3beta-HSD), cytochrome P450 17alpha-hydroxylase/c17, 20-lyase (P450c17), and aromatase in embryonic prosencephali. Our results indicated that 3beta-HSD mRNA levels were sexually dimorphic and developmental age-dependent. In particular, 3beta-HSD mRNA levels were higher in females than in males at E7, whereas, this dimorphism was reversed at E9 and E15. In females, the relative levels of 3beta-HSD mRNA were highest at E7, whereas, in males they were significantly higher at E9 and E15 than at E7 and at E11. This sexual dimorphism was a peculiar feature of the prosencephalon, it could not be observed before gonadal sexual differentiation and it was not paralleled by a dimorphism in the brain content of progesterone. The level of mRNA coding for P450scc and for P450c17 did not show obvious developmental- or sex-related variation. Aromatase mRNA varied as a function of the embryonic age but not of the sex. These results, taken together, are suggestive of a potential role of some neurosteroidogenic enzymes in the development of quail brain and suggest that sexual differences in the hormonal environment may occur during brain development.
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PMID:Sex- and age-related variation in neurosteroidogenic enzyme mRNA levels during quail embryonic development. 1829 19