Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.3 (HSD)
 3,464 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have examined the effects of IL-6 and bFGF on growth and reductive 17 beta-hydroxysteroid dehydrogenase (17-HSD) activity in a human ovarian cell line A2780 under defined culture conditions (standard RPMI (GM+PHR), phenol red-free RPMI (GM-PHR), or GM-PHR supplemented with 17 beta-estradiol). In media GM+PHR, neither factor influenced terminal cell density. However there was a 2-fold stimulation in 17-HSD activity (E1- > E2) in the presence of IL-6 and a 3-fold increase in the presence of bFGF. Cells cultured GM-PHR showed a 25-30% reduction in terminal cell density compared with cells cultured GM+PHR, and addition of factors resulted in a modest reduction (10-15%) in cell growth, with no detectable changes in 17-HSD. Addition of 17 beta-estradiol to GM-PHR cultures increased terminal cell density by up to 3-fold, with no effect on 17-HSD, and when combined with IL-6 or bFGF, a synergistic effect on growth was observed, but 17-HSD levels were unchanged. Our results suggest that cultured ovarian tumour cells respond to paracrine factors likely to be present in the ovary in vitro, and there is a possible interactive effect of estromedins with these factors.
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PMID:Growth factor regulation of 17 beta-hydroxysteroid dehydrogenase activity in a human ovarian cell line: modulation by 17 beta-estradiol. 823 11

Many years have passed since domestic water fluoridation was adopted to reduce the incidence of caries in developed countries; however, since there is an additional dose of fluorides ingested with foods and drinks prepared with such waters, the problem has emerged of possible adverse effects on health associated to them, so that in some countries fluorine integrator selling is allowed only with preventive medical prescription. Owing to the affinity for calcifited tissues, fluorine has a powerful effect on bone cellular order (mediated by growth factors' upregulation system IGF-2, TGF-beta, PDGF, bFGF, EGF, BMP-2 and PTH), on function and length, since it can provoke chronic joints-pain, ligaments-calcification, osteosclerosis. Moreover, sodium-fluoride may cause adverse effects on testicular activity (connected to oxidative-stress depending on increased activity of peroxidases and catalases) due to inhibition of 2 androgenesis-regulator enzymes DELTA(5)b-HSD and 17beta-HSD. Furthermore, insoluble gut formed calcium-fluoride may be responsible for hypocalcemia inducing a secondary hyperparathyroidism with bone matrix resorption, osteoporosis, osteomalacia and, perhaps, lowered level of phosphorus. At encephalic level, then, high doses of fluorine cause the onset of neurological symptoms and of a decreased spontaneous motor activity due to a reduction in the number of nicotinic acetylcholine receptors. Nevertheless, epidemiological studies about fluoride toxicity have established that such oligoelement may be safely used at odontoiatric dosages.
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PMID:[Fluoride toxicity]. 1510 74