Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.1.1.3 (
HSD
)
3,464
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objectives:
The aim of this study was to evaluate whether the gelatinase production ability of
Enterococcus faecalis
provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations.
Materials and methods:
Standardized dentin discs were randomly divided into five groups (
n
= 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (
n
= 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of
E. faecalis.
After incubation of the discs with the bacterial suspensions aerobically for 48 h,
XTT
assay was conducted for bacterial adherence evaluation. Data were statistically analyzed by ANOVA and Tukey's
HSD
tests (
p
= .05).
Results:
Gelatinase-producing
E. faecalis
adhered to dentin was significantly more than gelatinase-deficient
E. faecalis
in all test groups (
p
< .05). Adherence to CHX-treated dentin was lower than to the surfaces treated with other irrigants, alone or in combination (
p
< .05). These differences were significant except for comparisons with QMix for gelatinase-producing bacteria (
p
< .05).
Conclusions:
Gelatinase production of
E. faecalis
may be an important factor for bacterial adhesion. The addition of CHX to the irrigation regimen resulted in fewer adhered bacteria to dentin. QMix was not as effective as CHX in terms of bacterial adhesion prevention.
...
PMID:The effect of gelatinase production of
Enterococcus faecalis
on adhesion to dentin after irrigation with various endodontic irrigants. 2864 25
