Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.28 (lactic acid dehydrogenase)
 476 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Pseudomonas putida grew at the same rate with the same molar growth yield on D-, L, or DL-lactate as the sole source of carbon for growth. D- and L- lactate were utilized simultaneously and at the same rate when the organism was grown on DL-lactate (ratio of D isomer to L isomer of 1:1). Growth on either isomer alone, or in combination, caused the induction of both a D-lactate, and an L-lactate dehydrogenase. Both enzymes were particulate and used dichlorophenolindophenol, or oxygen, but not NAD, as electron acceptor, and were inhibited by cyanide when oxygen was the electron acceptor. The pH optimum for the D-lactate dehydrogenase was about 6.5, and for the L-lactate dehydrogenase was about 8.0. The D-lactate dehydrogenase was more heat-sensitive than the L-lactate dehydrogenase. The stoichiometry of both enzyme reactions was the same with 2 mol of lactate dehydrogenase. The stoichiometry of both enzyme reactions was the same with 2 mol of lactate being oxidized by 1 mol of oxygen to form 2 mol of pyruvate. No lactate racemase was detected in the cell extracts.
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PMID:Metabolism of D- and L-lactate by Pseudomonas putida. 61 7

Activity of D-lactate dehydrogenase (D-LDH) was shown not only in cell extracts from Megasphaera elsdenii grown on DL-lactate, but also in cell extracts from glucose-grown cells, although glucose-grown cells contained approximately half as much D-LDH as DL-lactate-grown cells. This indicates that the D-LDH of M. elsdenii is a constitutive enzyme. However, lactate racemase (LR) activity was present in DL-lactate-grown cells, but was not detected in glucose-grown cells, suggesting that LR is induced by lactate. Acetate, propionate, and butyrate were produced similarly from both D- and L-lactate, indicating that LR can be induced by both D- and L-lactate. These results suggest that the primary reason for the inability of M. elsdenii to produce propionate from glucose is that cells fermenting glucose do not synthesize LR, which is induced by lactate.
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PMID:Presence of lactate dehydrogenase and lactate racemase in Megasphaera elsdenii grown on glucose or lactate. 843 52

The effect of sodium acetate was studied on the change of the growth yield, the production of L- and D-lactic acid, and the activity of lactate dehydrogenases (LDHs; L-lactate dehydrogenase [EC 1.1.1.27, L-LDH] plus D-lactate dehydrogenase [EC 1.1.1.28, D-LDH]), fructose-1, 6-bisphosphate aldolase [EC 4.1.2.13, FBP-aldolase], and phosphofructokinase [EC 2.7.1.11, PFK] of Lactobacillus sakei NRIC 1071(T) and Lactobacillus plantarum NRIC 1067(T). The growth yield of L. sakei NRIC 1071(T) was increased 1.6 times in the presence of sodium acetate compared with its absence. The activity of LDHs in L. sakei NRIC 1071(T) and L. plantarum NRIC 1067(T) was retained longer under the addition of sodium acetate in the reaction mixture. As a result, these strains produced much more lactic acid in the presence of sodium acetate compared with its absence. Furthermore, the activity of L-LDH in L. sakei NRIC 1071(T) cultivated in the presence of sodium acetate increased three times or more compared with the activity of the cells cultivated in its absence. Consequently, the type of stereoisomers of lactic acid produced by L. sakei shifted from the DL-type to the L-type because the ratio of L-lactic acid to D-lactic acid produced became larger with the addition of sodium acetate to culture media. This phenomenon was not observed in L. plantarum NRIC 1067(T). Further, the participation of lactate racemase is discussed from the viewpoint of the production of D-lactic acid by L. sakei.
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PMID:The effect of sodium acetate on the growth yield, the production of L- and D-lactic acid, and the activity of some enzymes of the glycolytic pathway of Lactobacillus sakei NRIC 1071(T) and Lactobacillus plantarum NRIC 1067(T). 1246 5

The effect of sodium acetate on the production of stereoisomers of lactic acid produced by Lactobacillus sakei NRIC 1071(T) and other lactic acid bacteria was studied. L. sakei NRIC 1071(T) started producing L-lactic acid at the early logarithmic phase and d-lactic acid at the late logarithmic phase. The activity of L-lactate dehydrogenase [EC 1.1.1.27, L-LDH] from the resting cells of L. sakei NRIC 1071(T) appeared at the early stage of the logarithmic phase during the growth, and the activity of D-lactate dehydrogenase [EC 1.1.1.28, D-LDH] at the late stage of the logarithmic phase. The resting cells and cell-free extracts of L. sakei NRIC 1071(T) did not produce DL-lactic acid from L- or D-lactic acid. Stained bands of L-LDH and D-LDH appeared in the cell-free extracts from the cells of L. sakei NRIC 1071(T). Consequently, L. sakei conclusively produced L- and D-lactic acid by the action of L-LDH and D-LDH. This finding leads to the conclusion that lactate racemase [EC 5.1.2.1] does not exist in this strain. When the specific activity of LDHs (the total activity of L-LDH plus D-LDH) from the cells cultivated in the presence of sodium acetate is compared with that cultivated in its absence, the ratio of the activity between the cells cultivated in the former condition and those in the latter fell from 1.7 on the cell-free extracts to 1.3 on the preparation of the QAE-Toyopearl 550c chromatography. This result indicates that the amount of LDHs in the cells of L. sake NRIC 1071(T) cultivated in the presence of 50 mM sodium acetate was much more than that in the cells cultivated in the absence of sodium acetate. The shift of the type of stereoisomers of lactic acid from the DL-type to the L-type is discussed in the case of L. sakei strains.
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PMID:The effect of sodium acetate on the activity of L- and D-lactate dehydrogenases in Lactobacillus sakei NRIC 1071(T) and other lactic acid bacteria. 1268 66