Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:1.1.1.28 (
lactic acid dehydrogenase
)
476
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The intracellular expression of the B subunit of cholera toxin (
CTB
) was first achieved in Lactobacillus paracasei LbTGS1.4 with an expression cassette including the P25 promoter of Streptococcus thermophilus combined with the translation initiation region from the strongly expressed L. pentosus
D-lactate dehydrogenase
gene (ldhD). Secretion of
CTB
was next attempted in L. paracasei LbTGS1.4 and L. plantarum NCIMB8826 with four different signal sequences from exported proteins of lactic acid bacteria (Lactococcus lactis Usp45 and PrtP, Enterococcus faecalis unknown protein and S. pyogenes M6 protein). Host-dependent secretion of
CTB
was clearly observed: whereas none of the secretion cassettes led to detectable
CTB
in the extracellular fraction of L. paracasei LbTGS1.4, secretion of
CTB
molecules was clearly achieved with three of the selected signal sequences in L. plantarum NCIMB8826.
...
PMID:Production of cholera toxin B subunit in Lactobacillus. 985 Oct 32
