Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.27 (lactate dehydrogenase)
 29,211 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Vertical starch gel electrophoresis was used to resolve proteins encoded by 18 gene loci in ascaridoid nematodes. Estimates of genetic variability were made from population samples of the dog ascarid (Toxocara canis), cat ascarid (Toxocara cati), and the horse ascarid (Parascaris equorum). Levels of polymorphism and mean heterozygosity were high, which is not consistent with the hypothesis that the intestinal environment selects for monomorphism among endoparasites. Most observed allele frequencies conformed to Hardy-Weinberg equilibrium expectations as tested by chi2 goodness-of-fit, suggesting that the proteins evaluated are inherited in a Mendelian fashion and that these nematodes are mating at random. Subunit structures of the following enzymes, deduced from electrophoretic phenotypes of heterozygotes, corresponded to those of vertebrates: lactate dehydrogenase; malate dehydrogenase; 6-phosphogluconate dehydrogenase; phosphoglucomutase; esterase D; peptidase B; peptidase D; and mannose-6-phosphate isomerase. This observation substantiates the conservative nature of polypeptide subunit number across phylogenetically diverse groups of organisms.
 ...
PMID:Biochemical polymorphism in Parascaris equorum, Toxocara canis and Toxocara cati. 293 2

The specific activity of several red cell enzymes was studied in a pair of monozygotic twins, one of whom presented acute myeloblastic leukemia. When the intrapair variation of these twins was compared with that of a series of nine normal twins, a significant decrease in phosphoglycerate kinase, diphosphoglycerate mutase, pyruvate kinase, lactic dehydrogenase, adenylate kinase and glucose-6-phosphate dehydrogenase activities and an increase in 6-phosphogluconate dehydrogenase activity were demonstrable for the leukemic twin. The heat stability of the leukemic proband's pyruvate kinase at pH 8.0 and 56 degrees C was less than that of the normal twin, suggesting an acquired qualitative disorder.
 ...
PMID:Acquired erythroenzymopathy in a monozygotic twin with acute myeloid leukemia. 294 2

Serum protein (albumin, haptoglobin, ceruloplasmin, transferrin and group-specific component), haemoglobin, and red cell enzyme (glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, acid phosphatase, esterase D, adenylate kinase, glyoxalase I, phosphoglucomutase, lactate dehydrogenase, malate dehydrogenase, phosphohexose isomerase and superoxide dismutase) polymorphisms were studied among the Bengali Muslims of Bangladesh. In general, the gene frequencies of the polymorphic systems were similar to those in West Bengal and Assam. There appears to be a relatively strong Mongoloid influence in the present population as evidenced by the presence of HbE and TfDChi, higher frequencies of Hp1 and GcIF, and a lower AK2 frequency.
 ...
PMID:Blood genetic markers in Bengali Muslims of Bangladesh. 295 66

Haptoglobin, phosphoglucomutase 1 and 6-phosphogluconate dehydrogenase gene frequencies have been found for some Castillian provinces. The NADH diaphorase I, superoxide dismutase, lactate dehydrogenase, soluble oxaloacetate transaminase, soluble malate dehydrogenase, carbonic anhydrase I and carbonic anhydrase II are non-polymorphic red cell enzymes in Caucasoids and we have verified this in Castillian samples.
 ...
PMID:Some genetic markers in Castillian populations. 295 94

Hepatocytes were prepared from 15 degrees C acclimated catfish (Ictalurus punctatus) and maintained in primary culture for 20 days on biomatrix at 7, 15, and 25 degrees C without hormones or serum to determine if cells can directly adapt to temperature. Specific activities of cytochrome-c oxidase, NADH-cytochrome c reductase, citrate synthase, and glucose-6-phosphate dehydrogenase showed acclimatory rate compensation (7 greater than 15 greater than 25 degrees C cultured); 6-phosphogluconate dehydrogenase had activity changes of 15 greater than 7 greater than 25 degrees C cultured; activity of lactate dehydrogenase occurred in the series 7 greater than 15 = 25 degrees C. Protein synthesis of freshly isolated hepatocytes from catfish acclimated to the three temperatures exhibited acclimatory rate compensation. In contrast, protein synthesis of cultured hepatocytes occurred in the series 15 greater than 25 greater than 7 degrees C cultured. Protein degradation was highest at 25 degrees C followed by cells at 15 and 7 degrees C. Cultured hepatocytes showed incomplete temperature acclimation in vitro by way of enzyme activity changes and of protein synthesis. This suggests that some factor(s), such as hormones, is probably necessary to mediate the full temperature-acclimation process.
 ...
PMID:Can cultured teleost hepatocytes show temperature acclimation? 300 35

Fifteen isolates of blood containing Plasmodium vivax were collected from hospital in-patients in Rangoon and the schizonts were harvested for starch gel electrophoresis of the following parasite isoenzymes--glucose phosphate isomerase (GPI) (EC.5.3.1.9), NADP-dependent glutamate dehydrogenase (GDH) (EC.1.4.1.4), lactate dehydrogenase (LDH) (EC.1.1.1.27) and 6-phosphogluconate dehydrogenase (6PGD) (EC.1.1.1.43). Variation was found only in GPI. The other three isoenzymes appeared to be invariant in all the isolates. Forms of GPI were recorded in two isolates of P. vivax which differed from those reported for P. falciparum; these new forms were designated GPI-4 and GPI-5.
 ...
PMID:Isoenzyme variation in schizonts of Plasmodium vivax from Burma. 301 88

Within the uterine glands, the following enzymes were demonstrated by histochemical methods after 30, 58, 80, 100, and 110 d of pregnancy, respectively: beta-N-acetyl-hexosaminidase, beta-galactosidase, beta-glucuronidase, alpha-mannosidase, acid phosphatase, alkaline phosphatase, esterases, cytochrome oxidase, 5-nucleotidase, leucine aminopeptidase, adenosine triphosphatase, diaphorases (NADH, NADPH), glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, succinate dehydrogenase, isocitrate dehydrogenase (NAD, NADP), beta-hydroxybutyrate dehydrogenase, glycero-3-phosphate dehydrogenase, NAD-glycero-3-phosphate dehydrogenase, glutamate dehydrogenase (NAD, NADP), lactate dehydrogenase. The results show that the activities of G-6-PDH, 6-PGDH, and cytochrome oxidase increase within secreting cells during the 2nd half of pregnancy. The activities of the other enzymes remained almost unchanged during the period of investigation. The description of our results distinguishes between gland neck, middle, and distal part of the secretory unit, respectively. In general, the enzyme activities are similar within the middle and distal gland segments, but lower in the epithelia of the neck region. The activity of dehydrogenases was medium to intensive within the middle and distal gland segments, but only low to medium within the neck portion. Of the hydrolases, the acid phosphatase, ATPase, leucine aminopeptidase, and beta-galactosidase demonstrated an intensive activity within activity secreting cells. The enzyme activities of the gland epithelia are compared with these of the uterine surface epithelia and the histochemical results are discussed in context with their significance in histiotrophic nutrition.
 ...
PMID:[Enzyme histochemistry of the pig placenta. III. Histotopics of enzymes in the uterine epithelium]. 309 49

1. Following acclimation of channel catfish to a reduction in temperature from 25 degrees to 15 degrees C, there were approximately two-fold increases in liver mass, cell size, total protein, and total enzyme activity, relative to activity per milligram of protein and per gram wet weight of tissue, indicating tissue hypertrophy. There was no change in either total liver DNA content or protein concentration per gram weight. 2. Green sunfish, unlike catfish, showed virtually no change in liver mass following cold acclimation. However, sunfish showed a net increase in total liver protein content and an increase in protein concentration. The increase in protein content was balanced by a reciprocal and equivalent decrease in glycogen content. Consequently, liver mass was maintained. 3. During cold acclimation both catfish and sunfish showed an increase in ventricular heart mass and protein content, but no change in protein concentration. 4. The activities of several enzymes were measured in liver from 15 degrees C and 25 degrees C steady-state-acclimated catfish and at intervals following transfer from 15 degrees to 25 degrees C and from 25 degrees to 15 degrees C. Total tissue enzyme activity showed positive compensation which correlated with the change in liver mass and protein content. Specific activities based on protein and on wet weight showed dissimilar acclimatory patterns. Two enzymes - cytochrome oxidase and lactate dehydrogenase - showed inverse compensation in specific activity but positive compensation in total activity. Citrate synthase, glucose-6-phosphate-dehydrogenase and 6-phosphogluconate dehydrogenase showed positive compensation in both specific and total activities. 5. The increase in tissue protein content or 'protein hypertrophy' occurred with cell hypertrophy in cold-acclimated catfish, while protein hypertrophy occurred as an increased protein concentration without cell hypertrophy in sunfish. This phenomenon is considered adaptive in that it permits a compensatory increase in the total enzymatic capacity of a tissue. The two-fold increases in total enzyme activities, superimposed on either an increase or decrease in specific activity, suggest that two biochemical mechanisms may be operative during cold-induced liver hypertrophy, one effecting a specific step in protein translation at a point common to the synthesis of all proteins and a second targetted pretranslationally, i.e., transcriptional regulation.
 ...
PMID:Cold-acclimation-induced protein hypertrophy in channel catfish and green sunfish. 317 Aug 25

The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharose. This technique also gave purified lactate dehydrogenase. Both enzymes showed single bands in SDS polyacrylamide gel electrophoresis.
 ...
PMID:Purification of human 6-phosphogluconate dehydrogenase from human haemolysate with chromatography on an immobilized dye as the essential step and use of automation. Simultaneous purification of lactate dehydrogenase. 323 Jan 14

Muscle biopsies from six horses with clinical histories of muscle atrophy, muscle tremors, myopathic symptoms, unsteadiness of pelvic limbs and progressive ataxia were examined. Muscle biopsies were studied with enzyme histochemical techniques to evaluate the diagnostic values of these methods in cases suspected of suffering from neuromuscular disorders. Hypertrophy, atrophy, fibre splitting, waxy degeneration, phagocytosis and necrosis were seen in haematoxylin eosin stained sections of the different cases. Fibre type predominance and fibre type grouping were seen in the calcium ion stimulated myosine ATP-ase (Ca-ATP-ase) stained sections of some cases. 'Moth-eaten fibres' were demonstrated in three cases by staining with NADH: nitro blue tetrazolium oxidoreductase (NADH-TR), succinate dehydrogenase (SDH), NADH dependent malate dehydrogenase, cytochrome c oxidase and by lactate dehydrogenase. The catabolic enzymes, acid phosphatase (ACP) and 5'-nucleotidase were active in cases with fibre phagocytosis. The oxidative part of the pentose phosphate pathway in myopathic tissue seemed to be important in three cases, demonstrated by the increased activity of glucose-6-phosphate dehydrogenase (GPDH) and 6-phosphogluconate dehydrogenase (PGDH). The important feature of diseased horse muscle was that the pathohistochemical changes were exactly the same as in diseased skeletal muscles of humans. The application of tissue saving enzyme histochemical techniques can be recommended in the study of muscle tissue from horses suffering from suspected neuromuscular disorders.
 ...
PMID:Enzyme histochemistry on muscle biopsies as an aid in the diagnosis of diseases of the equine neuromuscular system: a study of six cases. 336 6


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>