Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.1 (alcohol dehydrogenase)
 9,284 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe a kinetic enzymatic method for ethanol in serum, based on the use of pyrazole, a competitive inhibitor for alcohol dehydrogenase (EC 1.1.1.1). The method is rapid and is easily automated for the Cobas Bio centrifugal analyzer. No specimen pretreatment is necessary and the total reaction time is 120 s. The standard curve is linear up to 140 mmol/L. The within-run CV was between 1.9% and 3.5%; the between-run CV ranged from 2.6% to 4.1%. Mean analytical recovery of ethanol added to serum was 100.2%. We compared results of the kinetic enzymatic method with those from a gas-liquid chromatographic (GLC) method and another commercial alcohol dehydrogenase method (TDx REA Ethanol; Abbott Diagnostics). Linear regression analysis gave the following equations: kinetic = 0.991GLC - 0.354 mmol/L (r = 0.992, n = 110) and kinetic = 0.998TDx - 1.741 mmol/L (r = 0.993, n = 70). No interference from methanol or isopropanol was seen.
 ...
PMID:Use of a competitive inhibitor in a kinetic enzymatic method for measuring ethanol in serum. 369 Aug 49

The most commonly used methods for the determination of blood alcohol are the Head-Space Gas Chromatography (HSGC) and the ADH-Method. Abbott offers now a REA-ethanol Assay for the AXSYM-System. The accuracy of the 3 analytical procedural were checked by a method comparison. This involved blood alcohol concentration of 347 serum samples being determined by means of all 3 methods. The deviation of the results of double analysis within the respective method, the difference of the values when using 2 measuring methods each (HSGC-ADH, ADH-AXSYM, HSGC-AXSYM) and the standard deviation of the measurements from HSGC-AXSYM and HSGC-ADH were determined. The REA Ethanol Assay, which was developed for diagnostic and therapeutic questions in cases of alcohol intoxication works with an accuracy which suffices entirely for this task designation. However, the fixed regulation for determining blood alcohol in forensic use cannot be observed fully.
 ...
PMID:[The accuracy of blood alcohol determination with head-space GC, ADH and REA ethanol assay for the AXSYM system--a comparison of the methods]. 892 74