Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:1.1.1.1 (alcohol dehydrogenase)
 9,284 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Major soluble proteins of grapevine ripe berries were extracted from six different cultivars including non vinifera, with trichloroacetic acid acetone and resolved in two-dimensional electrophoresis (2-DE) gels. About three hundred spots were detected on the 2-DE map after colloidal blue staining. From 2-DE map of cv. Gamay mesocarp, 67 proteins were identified (p > 0.95) using matrix-assisted laser desorption/ionization-mass spectrometry analysis. About 34%, 19%, and 13% of identified proteins play, respectively, a role in energy metabolism, defense, and stress response and primary metabolism. 2-DE analysis revealed considerable accumulation of dehydrin, invertase, and a putative transcription factor in the ripe fruit, in addition to pathogenesis-related proteins such as chitinase and thaumatin-like proteins previously documented as prevalent proteins in ripe berries. Actual translation of redundant transcripts of unclear function such as Grip31, Grip32, and Grip61 recently cloned in ripe grape berries was confirmed. The relative abundance of UDP-glucose pyrophosphorylase and vacuolar invertase strongly supported a key role of the apoplastic pathway of sugar loading during ripening. Comparative analysis shows that differences between cultivars were low, but different isoforms of alcohol dehydrogenase and of a transcription factor of hexose transporter were obvious in the six cultivars. Peptide mass fingerprinting suggests that the Adh isoforms would be Adh2/Adh6 or Adh2/Adh7 dimers and unambiguously shows that considerable deletion/insertion inside Adh7 are not cloning artifacts.
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PMID:Grape berry biochemistry revisited upon proteomic analysis of the mesocarp. 1473 Jun 82

SUMMARY Signal pathways involved in Solanum tuberosum-Erwinia carotovora ssp. carotovora(SCC3193) interaction were characterized. To this end, the concentration of several signal molecules implicated in plant defence such as ethylene (ET), jasmonates (JA) and salicylic acid (SA) were measured in potato plants treated by cell-free culture filtrates (CF) from E. c. carotovora(SCC3193). Furthermore, the presence of other potential signalling compounds such as cinnamic acid (CA) and related aromatic compounds was screened in the elicitor-treated plants. The activity of these signal compounds as inducers of defence-related genes such as drd-1 (a defence-related alcohol dehydrogenase), pinII (proteinase inhibitor II), chtB4 (basic chitinase) and chtA2 (acidic chitinase) was characterized. The results demonstrate that ET, JA and CA accumulate in potato tissues in response to CF. These signal molecules were shown to induce differential expression of drd-1, pinII, chtB4 and chtA2. Our data suggest that in addition to ET and JA, CA and possibly other aromatic compounds also may play a role in defence signalling in potato.
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PMID:Multiple defence signals induced by Erwinia carotovora ssp. carotovora elicitors in potato. 2056 78