Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

OUR FINDING THAT DNA BINDS TIGHTLY TO THE SURFACE OF CHRYSOTILE FIBERS (3MGO, 2SIO2, 2H2O) PROMPTED US TO TEST THE ABILITY OF THESE FIBERS TO ACT AS TRANSFECTION VEHICLES FOR THE UPTAKE OF PSV2-NEO DNA INTO MONKEY COS-7 CELLS. CELL MONOLAYERS (3 X 10(5) CELLS) WERE TRANSFECTED WITH PSV2-NEO DNA AS FOLLOWS: A) COPRECIPITATED WITH CALCIUM PHOSPHATE; B) ADSORBED TO 250 UG OF SONICATED CHRYSOTILE FIBERS (UICC SAMPLE AB "CANADIAN CHRYSOTILE"); OR C) IN BUFFERED SALINE. MICROSCOPY REVEALED CELLULAR UPTAKE OF CHRYSOTILE FIBERS OF 2-5 U LENGTH AND CONTACT OF THESE FIBERS WITH NUCLEI. CELLS WERE GROWN FOR 3 DAYS PRIOR TO ADDING ANTIBIOTIC G418 (400 UG/ML). AFTER 2 WEEKS, CULTURES WERE EXAMINED TO DETERMINE NUMBER OF G418-RESISTANT COLONIES OF 50 OR MORE CELLS. NO ANTIBIOTIC-RESISTANT COLONIES WERE OBSERVED IN CONTROL CULTURES TRANSFECTED WITH PSV2-NEO DNA IN SALINE ALONE. IN THE PRESENCE OF CHRYSOTILE FIBERS, TRANSFECTION FREQUENCY AVERAGED 0.7 X 10(-4), A VALUE APPROXIMATELY 1/3 THAT OBTAINED WITH CALCIUM PHOSPHATE. CHRYSOTILE FIBERS DID NOT TRANSFORM COS-7 CELLS TO G418 RESISTANCE IN ABSENCE OF PSV2-NEO DNA. FURTHER EXPERIMENTS DEAL WITH OPTIMIZING DNA UPTAKE AND EXAMINING THE FATE OF TRANSFECTED VECTOR DNA. THESE DATA SUGGEST THAT DNA UPTAKE MAY CONTRIBUTE TO THE CYTOTOXIC OR ONCOGENIC EFFECTS OF ASBESTOS FIBERS.
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PMID:ASBESTOS FIBERS MEDIATE THE UPTAKE OF DNA INTO PRIMATE CELLS IN CULTURE. 3026 Mar 40