Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Neural cell adhesion molecule
(
NCAM
) is a glycoprotein expressing on the surface of neurons, glial cells, bone cells and natural killer cells.
NCAM
plays an important role in the process of cell - cell adhesion and cell migration, and is also a model protein to study polysialic acid. In this paper,
NCAM
gene from mouse mammary gland cells (NMuMG) was cloned into eukaryotic expression vectors pcDNA3.1(+) and transfected into mutant Chinese hamster ovary cells ldlD-14. The stable transfection over-expressing
NCAM
was obtained through the
G418
selection and confirmed by Western blotting. Due to unique characters of ldlD-14 cells, carbohydrate chain of
NCAM
molecule can be easily manipulated with or without adding galactose in the serum free medium, and this modification can provide the basis for further studies on the effect of glycosylation on
NCAM
molecular function.
...
PMID:[Expression of neural cell adhesion molecule and modification of its N-glycan in ldlD-14 cells]. 2521 13
