Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Brain-derived neurotrophic factor
(
BDNF
) gene was cloned into retrovirous vector plasmid pLNCX to form the pLNC/
BDNF
. After packaging in PA317 cell line, the infectious particles were used to infect rat myoblast cell line L-6TG. After selection with
G418
, the L-6TG/
BDNF
cells were collected. Results from Southern blot show that bdnf gene has successfully integrated into the L-6TG genomic DNA. The expression of bdnf gene has been proved by Northern blot and Dot blot. The content of
BDNF
in the culture medium of L-6TG/
BDNF
was about 25 ng.10(-6) cells per 24 h per ml.
...
PMID:Expression of Brain-derived Neurotrophic Factor (BDNF) Gene in Rat Myoblast Cells. 1214 16
