Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Based on homologous recombination, recombinant plasmid pRKG was constructed by replacing the internal fragment of 18S rDNA of pRJ-5 with a copy of gamma-glutamylcysteine synthetase gene (GSH1) from the industrial brewing yeast strain G03 and a copy of G418 resistance gene (Kan) used as the dominant selection marker respectively. The fragment 18s rDNA::( Kan-GSH1) obtained through the PCR reaction was integrated to the chromosomal DNA of G03 strain, and recombinants were screened by G418 resistance. It was shown that the GSH content of beer fermented with the recombinant strain SG1 was 16.6% higher than that of G03, and no significant difference in routine fermentation parameters was found. To test the genetic stability, strains SG1 was inoculated into flasks and transfered continuously 5 times. The intracellular glutathione content of strain kept constant basically. It is an instructive attempt of genetically modifing industrial brewing yeast, as GSH1 was obtained from the host itself.
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PMID:[Improvement of beer anti-staling capability by genetically modifying industrial brewing yeast with high glutathione content]. 1825 39

Pichia pastoris is a common host organism for heterologous protein expression and metabolic engineering. Zeocin-, G418-, nourseothricin- and blasticidin-resistance genes are the only dominant selectable markers currently available for selecting P. pastoris transformants. We describe here new P. pastoris expression vectors that confer a hygromycin resistance base on the Klebsiella pneumoniae hph gene. To demonstrate the application of the vectors for intracellular and secreted protein expression, green fluorescent protein (GFP) and human serum albumin (HSA) were cloned into the vectors and transformed into P. pastoris cells. The resulting strains expressed GFP and HSA constitutively or inducibly. The hygromycin resistance marker was also suitable for post-transformational vector amplication (PTVA) for obtaining strains with high plasmid copy numbers. A strain with multiple copies of the HSA expression cassette after PTVA had increased HSA expression compared with a strain with a single copy of the plasmid. To demonstrate compatibility of the new vectors with other vectors bearing antibiotic-resistance genes, P. pastoris was transformed with the Saccharomyces cerevisiae genes GSH1, GSH2 or SAM2 on plasmids containing genes for resistance to Zeocin, G418 or hygromycin. The resulting strain produced glutathione and S-adenosyl-L-methionine at levels approximately twice those of the parent strain. The new hygromycin-resistance vectors allow greater flexibility and potential applications in recombinant protein production and other research using P. pastoris.
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PMID:Hygromycin-resistance vectors for gene expression in Pichia pastoris. 2482 43