Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A recombinant retroviral vector pNTK expressing the "suicide" gene herpes simplex virus thymidine kinase (HSV-TK) gene was constructed. The recombinant vector was packaged by PA317 cells and viral supernatant was used to infect the human pancreatic carcinoma cell line PC-2. After selection in G418, resistant colonies were identified and isolated. Cytotoxic effects of the non-toxic prodrug Acyclovir (ACV) or Ganciclovir (GCV) to the NTK transformant PC-2 cells was more than 90%, while that of the control PC-2 cells was less that 10%. Furthermore, the "bystander effect" of HSV-TK on PC-2 cells was also observed.
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PMID:[Gene therapy of human pancreatic carcinoma by recombinant retroviral vector expressing herpes simplex virus thymidine kinase gene]. 873 88

A recombinant retroviral vector expressing antisense c-myc was constructed. The recombinant virus DNA was packaged with packaging cell line PA317 cells by the calcium phosphate coprecipitation method. The virus supernatant was used to infect human pancreatic carcinoma cell line PC-2. After selection with G418, resistant colonies were obtained. Stable expression of retrovirus in both PA317 and PC-2 cells were confirmed by Northern blot hybridization. A down-regulation of endogenous c-myc was found in PC-2 cells infected with antisense c-myc construct. It was demonstrated that the antisense c-myc did inhibit the cell growth rate and 3H-TdR incorporation rate of PC-2 cells. The ability of colony formation in soft agar and tumorogenicity in nude mice of PC-2 cells were significantly suppressed by the antisense c-myc. The results implicate that recombinant retroviral vector containing antisense c-myc could inhibit target gene expression and partly reverse malignant phenotype of pancreatic adenocarcinoma cells.
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PMID:[Reversal of malignant phenotype of cells of human pancreatic adenocarcinoma cell line by recombinant retroviral vector expressing antisense c-myc]. 876 33