Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new aminoglycoside resistance gene (aphA1-
IAB
) confers high-level resistance to neomycin. The sequence of aphA1-
IAB
is closely related to aphA1 found in the transposons Tn4352, Tn903 and Tn602. For example, aphA1-
IAB
differs from aphA1-903 at five nucleotides that result in four amino acid replacements. The enzyme encoded by aphA1-
IAB
has a significantly higher turnover number with neomycin, kanamycin and
G418
as substrates than does the aphA1-903 enzyme. A parsimonious phylogenetic tree suggests that aphA1-
IAB
evolved from an ancestral form that is closely related or identical to the aphA1 found in Tn903. The excess of replacement substitutions over silent substitutions in aphA1-
IAB
, as well as its convergence toward aphA3 from Staphylococcus aureus, is indicative of selective evolution. Our hypothesis to explain these results is that aphA1-
IAB
evolved under the selective pressure of neomycin use in relatively recent times.
...
PMID:Evolved neomycin phosphotransferase from an isolate of Klebsiella pneumoniae. 166 55
