Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
IL-11
, a less identified cytokine, possesses some overlapping functions with IL-6 that are able to facilitate the growth and antibody secretion of B lymphocyte hybridomas. In this report, a DNA fragment encoding human
IL-11
was transduced into fusion partners (mouse myelomas Ag8.653 and SP2/0, and human lymphoblastoid cell line HF2) mediated by lipofection. The transfected cells selected with
G418
secreted
IL-11
constitutively over the range of 32.4 +/- 10.5 units/ml to 76.6 +/- 18.4 units/ml, which could be inhibited by an
IL-11
neutralizing MAb up to 80%. The fusion frequency of PBMC doubled, while that of LCLs displayed a 2.4- or 3.3-fold increase, when fused with the transfected fusion partners, respectively. The derived hybridomas from
IL-11
secreting fusion partner secreted 3 or 4 times as many immunoglobulins as that from its ancestor. Our data indicate that
IL-11
gene transfected fusion partners are improved cell lines for generation of human B lymphocyte hybridomas, and
IL-11
may contribute to the increased fusion frequency and antibody secretion of B lymphocyte hybridomas.
...
PMID:Improved fusion partners transfected with DNA fragment encoding IL-11 on generation of human B lymphocyte hybridomas. 1033 Nov 81
