Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In the present study, we examined the effect of interleukin-2 (IL-2) gene transfer into multidrug resistance (MDR) cancer cells on the therapeutic efficacy of MRK16. Human MDR ovarian cancer cells,
AD10
, were transduced with a bicistronic IL-2 retrovirus, Ha-IL2-IRES-Neo. The
G418
-resistant population, IL2-
AD10
, secreted IL-2 into the culture supernatant and did not form a tumor mass in nude mice. The IL2-
AD10
cells were more susceptible to the cytotoxicity of murine spleen cells than
AD10
cells in vitro. For examination of the effect of IL-2 gene transfer on the antitumor activity of MRK16 against P-glycoprotein-positive tumors, IL2-
AD10
cells were co-transplanted s.c. with
AD10
cells into nude mice in a ratio of 1:3, and the mice were treated with MRK16 on days 2 and 7. MRK16 markedly inhibited the growth of
AD10
cells mixed with IL2-
AD10
cells under conditions (0.3-1 microgram/body) where it showed only marginal effects on the growth of
AD10
tumors. These findings suggest that IL-2 gene transfer potentiates the antitumor activity of MRK16 against MDR tumors.
...
PMID:Combination therapy with antibody and interleukin-2 gene transfer against multidrug-resistant cancer cells. 943 86
