Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The cDNA of
PAI-2
mutants, PAI-2CD and PAI-2Q were inserted into eukaryotic expression vector, pcDNA3, producing pZLE-PAI2CD and pZLE-PAI2Q respectively. The
PAI-2
mutant expression plasmids were transfected into HeLa cells and the transfected cells expressing mutant proteins were selected by
G418
, Northern blot and ELISA assay. The ELISA assay showed that the expression level of
PAI-2
mutant protein was equal to that of wild type
PAI-2
in transfected cells. The biochemical characteristics of
PAI-2
mutant proteins were very similar to that of the wild type
PAI-2
. The results of crystal violet and MTT assays showed that the
PAI-2
mutants did not protect cells from the cytotoxic effects of TNF-alpha. The results of this study indicate that the protection of
PAI-2
from apoptosis induced by TNF-alpha is dependent upon the protein-protein interaction mediated by the CD-interhelical domain of
PAI-2
. The tTG-mediated interaction between
PAI-2
and cytosol protein might play a leading role in protecting from apoptosis induced by TNF-alpha.
...
PMID:The Protection Function of the PAI-2 from TNF-alpha is Dependent upon Its Protein Binding Domain. 1209 97
