Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
To investigate the biological function of p53 in colon carcinoma cells, a wild-type p53 expression plasmid under the control of the human cytomegalovirus promoter was stably transfected into the human colon adenocarcinoma cell line WiDr, which carries a mutation of the p53 gene at codon 273. Exogenous wild-type p53 transcripts were detected at various expression levels in 8 of 117
G418
-resistant clones. The growth rates of the wild-type p53+ clones in culture did not change significantly. The efficiency of colony formation in soft agar, however, was completely suppressed in two wild-type p53+ clones. This is the first to demonstrate the feasibility of stable transfection of the wild-type p53 gene under the control of non-inducible promoter in human colon cancer cells. The major alteration found was that wild-type p53+ cells which were incubated with anti-Fas IgM showed marked cytolysis with preferential over-expression of wild-type p53 accompanied by overexpression of a cyclin-dependent kinase inhibitor,
WAF1
, whereas the endogenous mutant p53 retained its expression level. The findings suggest that a Fas-initiated pathway is incidentally linked to a p53-dependent apoptotic pathway through the reconstituted wild-type p53 gene in WiDr cells. This model should help elucidating the additional role of the p53 tumor suppressor gene and the mechanism of apoptosis in colon carcinoma cells.
...
PMID:Induction of Fas-mediated apoptosis in p53-transfected human colon carcinoma cells. 747 11
To explore the functional role of p21(
WAF1
) gene on the proliferation of leukemia cell line K562, a p21(
WAF1
) retroviral expression vector was constructed. Mediated by FuGENE trade mark 6, p21(
WAF1
) was transfected into leukemia cell line K562, which was without p21(
WAF1
) expression. After selected in
G418
, K562 cell clones that expressed p21(
WAF1
) stabaly were isolated and named K562-p21(
WAF1
). The ectopic expression of p21(
WAF1
) mRNA and protein in K562 cells was identified by RT-PCR and Western Blot. The cell proliferaton was tested in liquid and soft agar culture after transfection. The cell cycle was tested by FCM. The expression of p21(
WAF1
) protein and mRNA could be detected in K562-p21(
WAF1
) cells. A strong inhibition of cell proliferation was observed in K562-p21(
WAF1
) cell clones cultured in liquid media as well as soft agar (P < 0.01). The cell number in G(0)/G(1) phase was remarkably increased. The findings showed that p21(
WAF1
) can inhibit the proliferation of leukemia cells, and it could be a potential target gene for leukemia gene therapy.
...
PMID:[p21(WAF1) Gene Transfection Inhibits Proliferation of Leukemia Cell Line K562] 1257 15
