Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A hamster DNA repair gene has been isolated by cosmid rescue after two rounds of transfection of an immortalized xeroderma pigmentosum (XP) complementation group D cell line with neomycin-resistance gene (neo)-tagged normal hamster DNA and selection with G418 and ultraviolet irradiation. The functional length of the sequence has been defined as 11.5 kilobase pairs by measurement of the region of overlap between two hamster DNA-containing cosmids, cloned by selection for the integrated neo gene, that are able to confer an increase in resistance to ultraviolet irradiation on two XP-D cell line but not on an XP-A line. Detailed molecular characterization of the hamster repair gene has revealed no obvious similarities to two human excision repair genes (ERCC1 and ERCC2) that correct repair-defective hamster cells but have no effect on XP cells. Hybridization analyses of normal human and XP cell genomic DNAs and mRNAs, using a cosmid-clone probe from which repeated sequences have been removed, show that homologues are present and expressed in all cases.
 ...
PMID:Molecular cloning and characterization of a mammalian excision repair gene that partially restores UV resistance to xeroderma pigmentosum complementation group D cells. 278 May 57

Xeroderma pigmentosum group E (XP-E) fibroblasts (XP95TO) were transformed with pSV3neo. Selection in medium containing G418 yielded 14 clones with extended life span. Following crisis, one clone was recovered that behaved in culture as an immortal cell line and was named XPET6/1. Expression of the SV40 large T antigen gene (Tag) and increased level of p53 were demonstrated by western analyses. Fingerprinting with 14 polymorphic microsatellite genetic markers confirmed that XPET6/1 originated from the parental strain XP95TO. XPET6/1 retained the sensitivity to killing by UV observed with the parental strain. Cell-free extracts from the immortal or the parental XP-E cells were deficient in excision, compared to extracts from HeLa or extracts from Tag-transformed XP variant fibroblasts. Complementation of XP-E extracts with XP-A, XP-D or XP-G extracts restored nucleotide excision activity to normal levels. XPET6/1 could prove a useful cell line for cloning of the XPE gene by functional complementation.
 ...
PMID:Generation and characterization of an immortal cell line of xeroderma pigmentosum group E. 953 81