Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB/c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10(-6) cells per microgram of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.
 ...
PMID:DNA-mediated gene transfer into epidermal cells using electroporation. 345 59