Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
As previously reported, transfer of RA538 into parental esophageal cancer EC8712 cell line induced its terminal differentiation and apoptosis. To further study the biological effects of this cDNA, an expression plasmid containing an insert of the putative coding fragment (about 0.3kb) of RA538 and neo resistance gene was constructed (designated pRA538-0.3-neo) and transferred into three different human cancer cell lines: EC8712, HL60 and GLC, a cell line derived from an
adenocarcinoma of the lung
. After selection in
G418
-containing culture media, the growth rate, 3H-thymidine incorporation rate, cell morphology, colony-formation in soft-agar, and heterotransplantation into nude mice of the surviving cell populations were tested. In situ hybridization verified the uptake and expression of the 0.3kb fragment of RA538 in these
G418
resistant cell populations. Significant reduction in growth rate and suppression of malignant phenotype were observed in all these cells in comparison with their parental cancer cell lines.
...
PMID:[Studies of the suppressive effect of cDNA RA538 on three human cancer cell lines]. 786 89
