Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Transposon tagging is a powerful tool that has been widely applied in several species for insertional mutagenesis in plants. Several efforts have aimed to create transfer-DNA (T-DNA) insertional mutant populations in
Brachypodium distachyon
, a monocot plant used as a model system to study temperate cereals, but there has been a lack of research aimed at using transposon strategies. Here, we describe the application of a maize (
Zea mays
)
Dissociation
(
Ds
) transposon tagging system in
B
distachyon
The
35S
::
AcTPase
cassette and
Ds
element were constructed within the same T-DNA and transformed into
B
distachyon
plants. The
Ds
element was readily transposed to other chromosomes or to the same chromosome under the function of
Activator
(
Ac
) transposase. Through homologous chromosome synapsis, recombination, and segregation, the
Ds
element separated from the
Ac
element. We selected stable
Ds
-only plants using
G418
and GFP assays and analyzed 241 T
0
lines, some of which were highly efficient at producing
Ds
-only progeny. Through thermal asymmetric interlaced PCR, we isolated 710 independent
Ds
flanking sequences from
Ds
-only plants. Furthermore, we identified a large collection of mutants with visible developmental phenotypes via this transposon tagging system. The system is relatively simple and rapid in comparison to traditional T-DNA insertion strategies, because once efficiency lines are obtained they can be reused to generate more lines from nontransposed plants without the use of time-consuming tissue culture steps.
...
PMID:An Efficient System for
Ds
Transposon Tagging in
Brachypodium distachyon
. 3086 34
