Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: DrugBank:EXPT01586 (
G418
)
2,237
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-
IL-24
fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-
IL-24
was cloned into expression vector pPIC9k; EGFRi-
IL-24
/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by
G418
and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-
IL-24
protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.
...
PMID:[Construction of eukaryotic expression vector of EGFRi-IL-24 recombinant gene]. 2048 26
