Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In some strains of cyanobacteria the composition of the light-harvesting antennae is determined by the color of available light. The mechanism of this chromatic adaptation involves the regulation of gene expression by red and green light and has been most studied in Fremyella diplosiphon (Calothrix sp. PCC 7601), a filamentous cyanobacterium for which there has been no reported means of genetic manipulation. We have constructed shuttle plasmids which can be efficiently mobilized by RP4 from Escherichia coli into F. diplosiphon and which can be recovered from transconjugant F. diplosiphon and returned to E. coli by transformation. The ability of these plasmids to replicate in F. diplosiphon is conferred by an 8.0-kb DNA fragment isolated from pFDA, a plasmid native to F. diplosiphon. To create these shuttle plasmids the 8.0-kb fragment was cloned into pJCF22, a mobilizable plasmid constructed from oriV and bom from pBR322, cat from pACYC184 and aphA from pACYC177.pJCF22 lacks sites for the restriction enzymes FdiI and II. Transconjugant F. diplosiphon containing shuttle plasmid pJCF62 are resistant to chloramphenicol and highly resistant to the aminoglycosides, G418 and neomycin. When aadA from the omega interposon was incorporated into a shuttle plasmid transconjugant F. diplosiphon could also be selected with streptomycin or spectinomycin. In F. diplosiphon shuttle plasmid pJCF62 replicates with a minimum copy number of seven. The oriV for replication in F. diplosiphon was localized to a 2.8-kb region within the cyanobacterial part of pJCF62. The presence on a shuttle plasmid of a single recognition site for FdiI reduced the efficiency of mobilization into F. diplosiphon by 5- to 10-fold. Restriction at this site was prevented when the E. coli donor strain in the mating contained the enzyme Eco47II methylase.
 ...
PMID:Construction of shuttle plasmids which can be efficiently mobilized from Escherichia coli into the chromatically adapting cyanobacterium, Fremyella diplosiphon. 823 95

Schwann cell cultures prepared from murine sciatic nerves (MuSN), rat sciatic nerves (RSN) and murine dorsal root ganglia (MuDRG) were transformed by the introduction of oncogenes (v-arc, c-Ha-ras, and v-mos). The transformation was carried out by infection with helper-free retroviruses containing the oncogene and the neomycin-resistant gene which were produced in psi-2 cells. After G418 selection and cloning of cells showing spindle shape morphology, six different transformed cell clones, i.e., MuSN-arc, MuDRG-arc, MuDRG-ras, MuDRG-mos, RSN-arc, and RSN-mos, were established. All of the clones were labeled with antibodies to the S-100 protein and the laminin, which are specific markers for Schwann cells. Fibronectin and vimentin were not detected in these cell clones, in contrast to fibroblasts as 3T3 and Rat-2. These cell clones have been maintained with characteristics of Schwann cells for over 18 months. When RSN-mos, one of the Schwann cell clones, and rat retinas were cocultured direct cellular interaction was demonstrated. Furthermore, by the addition of conditioned medium of the RSN-mos, a prominent activity promoting neurite outgrowth in PC12 pheochromocytoma cells was observed.
 ...
PMID:Establishment of Schwann cell lines by oncogene transfer. 1991 78