Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Interaction between the Edmonston or Nagahata strain of acute measles virus (MV) and the defective Biken strain of MV isolated from a patient with subacute sclerosing panencephalitis (SSPE) was examined by a cell fusion protocol. Biken-CV-1 cells nonproductively infected with Biken strain SSPE virus were fused with neomycin-resistant CV-1 cells. All the fused cells selected with the neomycin analog G418 expressed Biken viral proteins, as determined by an immunofluorescence assay. This procedure enabled the transfer of Biken viral genomes into cells previously infected with MV. In the fused cells coinfected by Biken strain SSPE virus and Edmonston or Nagahata strain MV, early MV gene expression was suppressed, as determined by immunoprecipitation with strain-specific antibodies. Maturation of Edmonston strain MV was also suppressed. When the coinfected fused cells were selected with G418, Biken viral proteins remained at a constant level for up to 7 weeks. Wild-type MV proteins gradually decreased to a barely detectable level after 4 weeks and became undetectable after 7 weeks. Immunofluorescence studies showed a steady decline in cells expressing wild-type MV proteins in the coinfected cultures. These results suggest that Biken strain SSPE virus dominantly interferes with the replication of wild-type MV. The possible mechanisms of dominant interference and the implication for evolution of a persistent MV infection are discussed.
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PMID:Subacute sclerosing panencephalitis virus dominantly interferes with replication of wild-type measles virus in a mixed infection: implication for viral persistence. 154 46

Apoptosis of bollworm cell line Hz-AM1 can be delayed by transient expression of AcNPV (Autographa californica Nuclear Polihedrosis Virus) p35 gene. Acp35Z, a p35 inactivated AcNPV by inserting with LacZ gene, cannot replicate in Hz-AM1 cells. However, the replication can be rescued by co-transfection with a plasmid containing AcNPV p35 gene. It is also realized that the transient expression of AcNPV p35 gene in Hela cells can put off cell apoptosis which is induced by adding actinomycin-D. Through co-transfection and G418 screening, two anti-apoptosis cell lines named Sf9-35 and Vero-35 are established by integrating AcNPV p35 and Neo expression cassette into the cell chromosomes. The Sf9-35 enhances the yield of budded virus of AcNPV, while the Vero-35 increases the propagation of measles virus.
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PMID:Functional study on baculovirus anti-apoptosis genes. 1457 82

Measles is still the leading cause of childhood death and is a vaccine-preventable disease globally. Even countries in which measles had been eradicated still remain at risk of importation from countries that have not yet eliminated the disease. Although two dose of immunization program with combined measles and rubella (MR) vaccine has been started since April 2006, there are still estimated 5-10 thousands measles cases annually in Japan. We introduced the Vero/hSLAM cell line for routine isolation of wild measles viruses in the WHO laboratory network. Vero/hSLAM cells express both CD46 and SLAM receptors and are highly sensitive to both vaccine and wild measles virus strains. Although Vero/hSLAM cell lines require an expensive antibiotics (G418) to maintain the transfection necessary for expression of the human SLAM receptor, we are trying to establish more simple, rapid and reliable diagnostic assays for measles virus infection.
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PMID:Current problems of measles control in Japan and Western Pacific Region. 1736 62

To improve the rescue efficiency of measles virus cDNA clone, the cell line that stably expressed the T7 RNA polymerase was established. Firstly, the T7 RNA polymerase gene was amplified by PCR and then the PCR product was inserted into pcDNA3 to obtain plasmid pcDNA3-T7. Vero cell was transfected with the plasmid and G418 was added to the cell 24h later to kill the cells without the plasmid. Western blotting analysis showed that the Vero/pcDNA3-T7 cell could express T7 RNA polymerase. To analyze the gene function of T7 RNA polymerase, the pT7IP-EGFP plasmid was transfected into the Vero/pcDNA3 T7 cell and EGFP was analized by fluorescence. The result suggested that T7 RNA polymerase expressed in the Vero/pcDNA3-T7 cell could transcribe the gene under control of the T7 promoter. Moreover, the minigenome PminiEGFP inserted reversely with report gene EGFP was established. After trans fection with the plasmid and infection with measles virus, EGFP was expressed, indicating the Vero/pcDNA3-T7 cell could rescue the minigenome.
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PMID:[Rescue of minireplicon by using the cell line stably expressing the T7 RNA polymerase]. 1789 37