Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: DrugBank:EXPT01586 (G418)
 2,237 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have constructed the expression vector pZipNexSV (x)-LMP by the recombinant DNA technology. The oncogene BNLF-1 of Epstein-Barr virus was inserted into retroviral vector pZip-NexSV (x) subjecting the BNLF-1 under the control of both promoter/enhancer sequence of MLV-LTR and EDL1 promoter of BNLF-1 gene itself. The fusion gene DNA was transfected into PA317 cells by electroporation. Ten of 54 G418-resistant colonies were allowed to grow up and the DNAs were checked by PCR. The results showed that 6 colonies carried the BNLF-1 gene. Northern hybridization and Western blotting to detect the transcription and translation of the BNLF-1 gene demonstrated that the fusion gene was expressed efficiently under the control of EDL1 promoter in PA317 cells.
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PMID:Cloning of the Epstein-Barr Virus Gene BNLF-1 and Its Expression in PA317 Cells. 1217 3