Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: CAS:99-94-5 (4-methylbenzoic acid)
 43 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Influence of cyclopropylethyl-containing benzoic acid amides on the aldehyde dehydrogenase activity of the rat liver mitochondria was investigated. Values of I50 were measured for each of the compounds. The character of kinetic behavior of these reversible inhibitors was studied. It is shown that 2,5-dichloro-4-methylbenzoic acid amides reveal partially uncompetitive type of inhibition relative to acetaldehyde, while 2,4-dichloro- and 2,4-dichloro-5-methylbenzoic acid amides reveal changes in the character of inhibition from uncompetitive to mixed one. Inhibition was partially competitive with regard to reaction NAD+ cofactor. Though the studied compounds induced inhibition of a certain type with respect to each of the substrates, but on the whole the mechanism promoting a decrease of the ALDH-1 reaction rate appears to be more complex.
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PMID:[Inhibition of aldehyde dehydrogenase activity in rat liver by cyclopropylethyl-containing benzoic acid amides]. 804 81

It was demonstrated from the results of kinetic analysis that acetaldehyde oxidation reaction, which is catalyzed by membrane isoenzyme forms of mitochondrial and microsomal aldehyde dehydrogenases, has the ordered Bi-Bi mechanism. An attempt was made to compare the active site structures of these two isozyme forms using cyclopropylethyl-containing benzoic acid amides which are the reversible inhibitors of the studied enzyme activity. The amides competitively inhibited each enzyme activity relative to acetaldehyde. The inhibition constant values were calculated. As to NAD+ partially competitive type of inhibition was observed for aldehyde dehydrogenase of the microsomes. At low amide concentrations the mitochondrial aldehyde dehydrogenase was inhibited uncompetitively. With increased concentration of the compounds over 75 microM the character of inhibition by 2.4-dichlorine- and 2.4-dichlorine-5-methylbenzoic acid amides changed to mixed one; and when using the amides of 2.5-dichlorine-4-methylbenzoic acid it changes for pseudoinhibition. The difference of kinetic behaviour of the studied compounds with aldehyde dehydrogenase membranes forms suggests the conformation similarity of the sites for binding acetaldehyde for the both enzymes and distinction of the sites for coenzyme binding.
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PMID:[Comparative characteristics of membrane forms of aldehyde dehydrogenase]. 886 10